Had the E2 blood test today (the one that I had insisted upon). It was only 355 after 5 days on Puregon. This is obviously not what it should be as they have now increased my dosage from 225IUI to 275IUI. What is normal? What doses do others take?

What should the estrogen level be? I recall my doctor saying something about wanting it in the thousands before EPU. I was hoping for EPU on Monday 25 Feb, they will be in my town doing EPU on M, T & W. IVF nurse said it should double daily… they will do another test on Friday and an ultrasound.  I am now really worried that there might not be any follicles to scan.